TRUPCR® Dengue Virus Serotyping Kit

TRUPCR® Dengue Virus Serotyping Kit

Dengue virus (DENV) is a mosquito-borne, positive-sense single-stranded RNA virus belonging to the Flaviviridae family and Flavivirus genus. It is primarily transmitted by Aedes aegypti mosquitoes and, to a lesser extent, Aedes albopictus, which also transmit chikungunya, yellow fever, and Zika viruses. DENV has an approximately 11 kb RNA genome and exists as four distinct serotypes (DENV-1 to DENV-4), each differing in its interaction with human antibodies. Although the serotypes share about 65% genomic similarity, genetic variation exists within each serotype. In India, DENV-2 has been the predominant serotype over the past five decades, while DENV-3 and DENV-4 have also been associated with outbreaks.

The TRUPCR® Dengue Virus Serotyping Kit is an in vitro nucleic acid amplification assay for the qualitative detection and typing of dengue virus serotypes 1, 2, 3, and 4 specific RNA from human serum or plasma collected from human patients with signs and symptoms consistent with dengue infection using qRT-PCR technique. The results from the TRUPCR® Dengue Virus Serotyping Kit must be interpreted within the context of all relevant clinical and laboratory findings. Internal control has been integrated into the kit to check PCR inhibition.

The TRUPCR® Dengue Virus Serotyping Kit is a one-step real-time reverse transcription PCR assay in which RNA templates are first reverse-transcribed to generate complementary cDNA strands followed by DNA polymerase-mediated cDNA amplification. During cDNA amplification in real-time PCR, the fluorescent signal is generated from the presence of an oligonucleotide probe specific to the target DNA sequence. The probe contains a fluorescent dye molecule on its 5’ end and a quencher molecule on its 3’ end. The probe hybridizes with one of the chains of the amplified fragment. During the synthesis of a complementary chain, Taq DNA polymerase which possesses 5'- 3' exonuclease activity cleaves the probe. As a result, the fluorescent dye and quencher dye are separated, and the total fluorescence of reaction volume increases in direct proportion to the number of amplicon copies synthesized during PCR. The fluorescent signal is measured in each cycle of the reaction.

Key Features:

• CDSCO Licensed
• Intended for simultaneous detection and differentiation of DENV-1, DENV-2, DENV-3, and DENV- 4 serotypes
• One-step Multiplex Real-Time RT-PCR technology
• Detects dengue virus serotypes from human serum or plasma.
• Integrated Internal Control to check PCR inhibition
• High analytical sensitivity with LoD of 1 copy/µL
• Compatible with major Real-Time PCR platforms including Applied Biosystems, QuantStudio, Rotor-Gene Q, Bio-Rad CFX96

Publications:

1. Mohanty M, Tripathy A, Mamidi P, Panda S, Ray A, Bhoi SK, Dey A, Chhabra G, Mohapatra D, Mandal MC, Santra D, Dwibedi B, Gupta K, Raghav SK, Chattopadhyay S, Mishra B. Comparison of Inflammatory Cytokine Responses in DENV-1 Monoinfection Versus Coinfection with DENV-4. Vector Borne Zoonotic Dis. 2026 Apr;26(4):211-219. doi: 10.1177/15303667251413443. Epub 2026 Jan 15. PMID: 41874271.
2. Khare K, Imran M, Ravi V ... Inter-host diversity associated with age, sex, and menstrual cycle modulates clinical manifestations in DENV-2 patients iScience, 2025; 28
3. Ravi V, Khare K, Mohite R, Mishra P, Halder S, Shukla R, et al. (2025) Genomic hotspots in the DENV-2 serotype (E, NS4B, and NS5 genes) are associated with dengue disease severity in the endemic region of India. PLoS Negl Trop Dis 19(4): e0013034.
   https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0013034
4. Pandey, Pooja & Ranjan, Rahul & Agarwal, Priti & Gupta, Rajiv & Pandey, A & Das, A & Yadav, Vishwanath. (2024). Dynamic trends of dengue fever serotypes in northern India: Exploring clinical manifestations, serotype dissemination, and the influence of mixed infections. Tropical Biomedicine. 41. 464-470. 10.47665/tb.41.4.007.
5. Gupta S, Shakya A, Mishra N, et al. (April 05, 2024) Investigating the Clinico-Demographic Characteristics of Dengue Fever and Its Seroprevalence at a Tertiary Care Hospital in Northern India. Cureus 16(4): e57640. DOI 10.7759/cureus.57640