Test Overview
The TRUPCR® MYD88 Mutation Detection Kit is an in vitro diagnostic test intended for the qualitative detection of L265P mutation in c.794T>C in myeloid differentiation factor 88 (MYD88) gene from tumor tissue DNA (fresh, frozen or formalin fixed paraffin-embedded tissue)/bone marrow or peripheral blood. Results are intended to aid the clinician in classifying patients among Lymphoplasmacyticlymphoma/Waldenstrom Macroglobulinemia (LPL/WM) and non-Hodgkin lymphoma and carry out treatment planning.
Principle and Procedure:
The MYD88 L265P mutation is a significant biomarker in various B-cell malignancies, particularly WM and certain subtypes of Diffuse Large B-Cell Lymphoma (DLBCL). In WM, this mutation is present in over 90% of cases, aiding in diagnosis and influencing treatment decisions. The TRUPCR® MYD88 Mutation Detection Kit is based on allele specific amplification and is achieved by ARMS PCR. The kit is designed to selectively amplify mutant specific sequences in samples that contain a mixture of wild-type and mutated DNA. The TRUPCR®MYD88 Mutation Detection Kit is a single tube kit where the detection is achieved in multiplex reaction using fluorescent probes labelled with FAM (L265P mutation in c.794T>C) and VIC/HEX (reference: a region of MYD88 gene without any known polymorphism/mutation).
Key Features:
- The kit allows highly sensitive and specific diagnosis of MYD88 Mutation in single run.
- The kit offers sensitivity to detect as low as 0.5% allele of MYD88 L265P.
- All-inclusive kit: The Assay includes all the PCR components including PCR Pre-mix / master mix for optimized results.
- Positive control is included in the kit for reliable results
Ordering Information:
| CAT. NO. | PRODUCT | CONTENTS |
|---|---|---|
| 3B1427 | TRUPCR® MYD88 Mutations Detection Kit | 48 Reactions |
| 3B1428 | TRUPCR® MYD88 Mutations Detection Kit | 96 Reactions |
