Test Overview
TRUPCR® Carbapenem Resistance Detection Kit is an in vitro nucleic acid amplification assay for th e qualitative detection and differentiation of the most prevalent carbapenemases gene families – bla KPC (KPC- Klebsiella pneumoniae carbapenemase), bla NDM (NDM-New Delhi Metallo-beta-lactamase), bla VIM (VIM-Verona integron-mediated metallo-beta-lactamase), bla OXA-48 (OXA-48-Oxacillinase-48) and bla IMP (IMP-Imipenemase metallo-beta-lactamase) on Real-Time PCR.
There is a multiplexing reaction running in parallel in two tubes to detect different targets with the help of three different dyes (FAM, Yellow/HEX/VIC & Orange/ROX/TEX Red).
TECHNICAL SPECIFICATIONS
- Sample Type – Pure Colonies, Perirectal or Rectal swabs, Sputum, BAL, Pus, Aseptic fluid, Bacterial Cultures
- Target Regions – Conserved region of blaKPC, blaNDM, blaVIM, blaOXA-48, and blaIMP gene
- Clinical Validation – Validated on more than 1000 clinical samples
- LoD: 500 CFU/ml
- Reaction Volume – 25 µl in each tube
- Internal Control integrated into the kit in order to check PCR inhibition
- Compatible Instruments – Applied Biosystems™ 7500 series, Applied Biosystems™ QuantStudio® 5, Rotor-Gene Q, Bio-Rad CFX96
About Carbapenem Resistance:
Carbapenem resistance is a major and an on-going public health problem globally. It occurs mainly among Gram-negative bacteria. Infections by such pathogens pose a serious threat to hospitalized patients due to resistance of all beta-lactam antibiotics as well as co-resistance to most other antibiotics, leaving very few treatment options which are associated with clinical and economic consequences. Culture is the reference method for detection, requires at least a turn-around time of between 2-3 days which will be critical in emergency and ICU patients when the time available for intervention is limited. New CLSI recommendations aim to speed up detection and improve treatment outcomes for Carbapenem resistance through use of a molecular diagnostic test.
Ordering Information:
| CAT. NO. | PRODUCT | CONTENTS |
|---|---|---|
| 3B289 | TRUPCR® Carbapenem Resistance Detection Kit | 48 Reactions |
| 3B290 | TRUPCR® Carbapenem Resistance Detection Kit | 96 Reactions |
Publications:
- Partha Guchhait, Nairita Choudhuri, et al. “Improved diagnostic stewardship in carbapenem-resistant Enterobacterales gene detection helps in early initiation of targeted therapy”
- Gaurav Verma, Subham Ravi Nayak, et al. “Detection of Carbapenem-Resistant Genes Among Clinical Isolates of Acinetobacter baumannii in a Tertiary Care Hospital”
